Recent vicariant and dispersal events affecting the phylogeny and biogeography of East Asian freshwater crab genus Nanhaipotamon (Decapoda: Potamidae)

The molecular phylogeny and biogeography of the East Asian freshwater crabs of the genus Nanhaipotamon (Decapoda: Brachyura: Potamidae) were studied, using two mitochondrial (16S rRNA and cytochrome oxidase I) and one nuclear (28S rRNA) markers, and correlated with various vicariant and dispersal events which have occurred in this region. The results showed Nanhaipotamon to be a monophyletic taxon with four clades which correspond to the topography of the coastal region of southeastern China and Taiwan Island. Mountains appear to play an important role in the distribution. The genus occurs only from east of the Wuyishan Range (Zhejiang and Fujian) and south of the Nanling Range (Guangdong) in southern China, and is also present west of the Central Range in Taiwan. The molecular and geological data suggest that Nanhaipotamon originated in an area between the Wuyishan and Nanling Ranges. In this area, the main and earliest cladogenesis occurred at ~4.8 million years ago (mya), with speciation probably taking place at around 4 mya. The molecular evidence strongly supports the recent invasion of the genus into Taiwan Island from northeastern Fujian, via the paleo-Minjiang River on the landbridge of Taiwan Strait. The presence of the genus in Dongyin Island, however, is through invasion from southeastern Zhejiang, during the Pleistocene glaciation period. Nanhaipotamon reached Taiwan and Dongyin Island at ~1.0 and 0.4 mya, respectively. A small population of Nanhaipotamon formosanum from Penghu Islands (Pescadores) in the central Taiwan Strait has a slightly different genetic constitution and suggests it is a relict of past Pleistocene glaciations.

Key words: Nanhaipotamon, Freshwater crabs, Phylogeny, 16S rRNA, COI, 28S rRNA, Vicariance, Dispersal